Non-Invasive Detection of Cancer Biomarker Using an Electrochemical Biosensor

Cancer remains a major global health burden, where early detection is critical for improving survival outcomes and reducing treatment costs. The identification of reliable molecular biomarkers at initial stages can substantially enhance diagnostic accuracy and support minimal invasive therapeutic interventions. In this study, we develop an electrochemical biosensing platform designed for cancer biomarker detection using impedance-based measurements. The biosensor integrates a graphene-modified screen-printed electrode functionalized with specific antibodies targeting cancer biomarker. The binding interactions were observed through variations in impedance via electrochemical impedance spectroscopy, offering a label-free and sensitive detection approach. Preliminary experiments demonstrated a strong logarithmic correlation between target biomarker concentration (1 – 100 ng/mL) and impedance response. These results demonstrate the promise of this developed graphene-based biosensor as a low-cost, economical option for early cancer diagnostics1-3.

1. Hansen, L.; Nagdeve, S.N.; Suganthan, B.; Ramasamy, R.P. An Electrochemical Nucleic Acid Biosensor for Triple-Negative Breast Cancer Biomarker Detection. Sensors 2024, 24, 5747.
2. Nagdeve, S.N., Suganthan, B. & Ramasamy, R.P. An electrochemical biosensor for the detection of microRNA-31 as a potential oral cancer biomarker. J Biol Eng 19, 24 (2025).
3. Nagdeve SN, Suganthan B, Ramasamy RP. Perspectives on the Application of Biosensors for the Early Detection of Oral Cancer. Sensors (Basel). 2025 Feb 27;25(5):1459.