P-155-R: Behind the Scenes: Creation of a Broadly Reactive E. coli HCP Detection ELISA Suitable for K Strain and B Strain Platforms
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Escherichia coli (E. coli) is a major expression platform to produce biopharmaceuticals. ELISAs remain the workhorse method of monitoring HCP (host cell protein) levels through the bioprocess, demonstrating HCP removal and consistency in manufacturing. Here we present the development and validation of a new E. coli HCP ELISA that has been qualified for monitoring workflows for both recombinant proteins (B-strains) and for plasmid production in GTx (K-strains).
