The T-SPOT® Discovery SARS-CoV-2 kit

The T-SPOT® Discovery SARS-CoV-2 kit

Biotech

Information

The T-SPOT Discovery SARS-CoV-2 kit has been developed to aid in the investigation of the T cell response to SARS-CoV-2 infection. The kit uses a modified ELISPOT assay where samples prepared from peripheral blood are stimulated in vitro by peptide pools from SARS-CoV-2. This re-stimulation allows SARS-CoV-2 specific T cells to be enumerated. The kit builds on Oxford Immunotec’s experience with TB diagnosis and the assessment of immune response to CMV in transplant patients, to apply the Company’s established, proprietary T-SPOT technology to the fight against COVID-19. While serology is able to detect antibodies to SARS-CoV-2 in the blood of some individuals after infection, little is currently known about how this confers immunity to COVID-19. The T-SPOT technology goes further than simple serology by interrogating the immune system’s T cell response and will enable research into the measurement of the strength of that response to SARS-CoV-2. The strength of this response may be linked to protection from reinfection.
Oxford ImmunotecOxford Immunotec has been developing and strengthening its expertise in T cell measurement for over 15 years since the introduction of its T-SPOT® technology (ELISPOT) in the field of TB diagnostics. The T-SPOT®.TB test is approved in over 60 countries, including US, EU, Japan and China. The research use-only T-SPOT Discovery SARS-CoV-2 assay uses this same T-SPOT technology to allow SARS-CoV-2-specific T cells to be detected and enumerated. The optimized antigen mix used in the test is based on SARS-CoV-2 structural proteins, with sequences of high homology to other coronaviruses removed. This allows the maximum breadth of the immune response to be measured, while minimizing cross reactivity. Oxford Immunotec’s single antigen-per-well approach also allows specific information about the immune response to different SARS-CoV-2 antigens to be gathered in parallel. T-SPOT technology can be automated using the T-Cell Select™ reagent kit to accommodate variations in scale - automation solutions are available for low, medium andhigh throughput settings. The T-Cell Select reagent kit also enables sample stability of 54 hours to be achieved, simplifying sample logistics.

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