Optimization of the HEK293T Suspension Cultivation With a DoE-Approach in Ambr® 15 Cell Culture

This study demonstrates that the Ambr® 15 Cell Culture system in combination with the DoE MODDE® software enables a systematic investigation of critical process parameters and rapid, high throughput process improvement and optimization. The results prove that the transition from shake flask to a scalable stirred bioreactor system can be accomplished very fast. A key next step is to use the identified HEK293T culture conditions to perform a DoE study with the Ambr® 15 to optimize viral vector production for cell and gene therapy applications. Please copy and paste the link below to access the poster.