ELISpot and FluoroSpot / Detecting cytokines at the single-cell level
Contract Research
Information
ELISpot and FluoroSpot build on familiar immunoassay principles to assess the frequency of antigen-specific cytokine-secreting cells. With its high sensitivity, it can detect low antigen-specific responses and be used to complement information from other functional assays, including intra-cellular cytokine staining (ICS) by flow cytometry and ELISA.
Fit-for-purpose ELISpot assay for the detection of IFNγ:
- Readily available for exploratory endpoints and can be validated for secondary and primary endpoints
- Validation performed on cryopreserved PBMC
- LOD established at 9 SFC/well and ULOQ at 661 SFC/well
FluoroSpot:
With FluoroSpot assays, analyte secreting cells are detected using antibodies labeled with fluorophores instead of enzymes, resulting in a one-step direct detection as compared to the two-step detection system of ELISpot. FluoroSpot also has the advantage to enable the analysis of multiple analytes in one well.
For more information: https://immunecarta.com/en/immune-monitoring-services-technology/elispot.php