ELISpot and FluoroSpot / Detecting cytokines at the single-cell level

Information

ELISpot and FluoroSpot build on familiar immunoassay principles to assess the frequency of antigen-specific cytokine-secreting cells. With its high sensitivity, it can detect low antigen-specific responses and be used to complement information from other functional assays, including intra-cellular cytokine staining (ICS) by flow cytometry and ELISA. Fit-for-purpose ELISpot assay for the detection of IFNγ: - Readily available for exploratory endpoints and can be validated for secondary and primary endpoints - Validation performed on cryopreserved PBMC - LOD established at 9 SFC/well and ULOQ at 661 SFC/well FluoroSpot: With FluoroSpot assays, analyte secreting cells are detected using antibodies labeled with fluorophores instead of enzymes, resulting in a one-step direct detection as compared to the two-step detection system of ELISpot. FluoroSpot also has the advantage to enable the analysis of multiple analytes in one well. For more information: https://immunecarta.com/en/immune-monitoring-services-technology/elispot.php