TCR antigen Profiling-TAP

Platform Technology

Information

TAP is used to identify T cell receptors and their cognate antigens by combining FACSorting and single cell sequencing capabilities. Briefly, CD8+ T cells are stained with oligo tagged MHC Class I and a panel of oligo tagged antibodies that are specific to cell surface proteins of interest. Antigen specific T cells are enriched by FACsorting. Subsequently the enriched T cells are subjected to single cell sequencing to obtain TCR, antigen and cell surface antibody specific readouts.

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