PRESENTATION: An Overview of Various Endotoxin Detection Tests

• Debating which techniques are most effective at detecting endotoxins swiftly and sensitively to prevent severe illness

• Field-effect transistor (FET) biosensors and aptamers as one of the most promising biosensors in recent years

• Endotoxin detection advancement including techniques such as molecular imprinting, quartz crystal microbalance (QCM), HPLC, and electrochemical approaches

• Discussing a new technique involving recombinant cascade reagent (rCR), which replicates the LAL reaction naturally without utilising horseshoe-derived blood cells

• Other promising methods for endotoxin detection that allow for quick, label-free and sensitive analysis - using the mass sensing capabilities of quartz crystal microbalance (QCM) and the highly specific binding sites created by molecular imprinting (MIP)

• Focus on an in vitro test called the Monocyte Activation Test (MAT) that gauges how human monocytes react to endotoxins making it an alternative where traditional methods cannot suffice

• The rolling droplets technique as one additional novel technique that detects amphiphiles at extremely low concentrations by using rolling droplets on micro structured surfaces