The role of phospholipases and their chemical inhibitor in modulating wound-induced JA biosynthesis

Abstract Description: Jasmonate (JA) biosynthesis is tightly regulated to promote rapid defense responses to insect herbivory while minimizing its negative impacts on plant growth. Plastid-localized phospholipases A1 (PLA1s) control the first step in JA biosynthesis. Through a series of physiological, pharmacological, genetic, and kinetic analyses of gene expression and hormone quantification, we demonstrated that this regulation occurs through a mechanism independent of gene transcription and translation to initiate the rapid JA burst in wounded leaves. From a chemical screen, we identified a potent inhibitor of wound-triggered JA biosynthesis (wjin1) that targets the PLA1s. Wjin1 demonstrated effectiveness at sub-micromolar concentrations against DEFECTIVE IN ANTHER DEHESCENCE 1. While effective in inhibiting JA-dependent wound responses in Arabidopsis and wound-elicited JA biosynthesis across diverse angiosperms, wjin1 did not interfere with normal plant growth or responses to other major plant hormones. Together, wjin1 represents the first plant PLA1 inhibitor of wound-induced JA biosynthesis and is a useful research tool. These and other works involving genetic manipulations and physiological characterization of the effector, as well as JA metabolism and signaling, will be presented.

Equity and Inclusion: We value diversity in our research by bringing together people with different backgrounds, skills, and experiences. This helps us look at science from many angles. We're committed to equity by making sure everyone has the same access to resources, opportunities, and recognition, no matter who they are or where they come from. We also focus on inclusion, building a collaborative and respectful space where everyone on the team feels valued and can share their ideas. This presentation will embody the culmination of the aforementioned efforts.