Temporal Profiling of Root Gravitropism: Correlating Physiological, Transcriptome, and Proteome Players - Lightning Talk

Combining RNAseq and tandem mass tag-labeled LC-MS/MS analyses, we resolved the transcriptomic and proteomic response of 10 days old Arabidopsis plant grown in the Biological Research In Canisters - Light Emitting Diode hardware aboard the International Space Station, with control samples on Earth. Our results indicated high discordance between the RNA transcripts and the protein expression, suggesting the prevalence of post-transcriptional regulation in microgravity or that RNA transcriptome might be a reflection of the plant's immediate response to microgravity stress, while the proteome represents a more stabilized, adaptive response.