Investigating the Biogenesis of Chloroplasts and Vacuoles in Plant Cells with Electron Tomography
Tuesday, June 25, 2024 2:15 PM to 2:35 PM · 20 min. (US/Hawaii)
Meeting Room 312
Cell Biology
Information
Electron tomography (ET) with nanometre resolution is especially useful for investigating
convoluted three-dimensional (3D) lamellar networks. Utilizing 3D-ET, we illustrate the thylakoid assembly and the underlying mechanisms involved in the developmental progression from proplastids or etioplasts to chloroplasts.
The Arabidopsis microspore is another excellent model for studying organelle dynamics. With their well-defined developmental stages, our whole-cell ET models showed dynamic and distinct vacuole distributions at six developmental stages, albeit with elusive mechanisms. More recently, we have successfully demonstrated the feasibility of advancing cryo-FIB and cryo-ET tools for identifying vacuoles in pollens at their closer-to-native state with an unprecedented resolution.
convoluted three-dimensional (3D) lamellar networks. Utilizing 3D-ET, we illustrate the thylakoid assembly and the underlying mechanisms involved in the developmental progression from proplastids or etioplasts to chloroplasts.
The Arabidopsis microspore is another excellent model for studying organelle dynamics. With their well-defined developmental stages, our whole-cell ET models showed dynamic and distinct vacuole distributions at six developmental stages, albeit with elusive mechanisms. More recently, we have successfully demonstrated the feasibility of advancing cryo-FIB and cryo-ET tools for identifying vacuoles in pollens at their closer-to-native state with an unprecedented resolution.
Day
6/25/2024
Event Format
Formats
In-Person Event
In-Person Program Elements
Concurrent Talk
