Electrified interfaces are ubiquitous in nature and play a central role in chemical analysis, impacting performance in chemical separations and sensing and a myriad of processes that are frequent targets of analytical methods (i.e., biological transformations that affect disease development and progression, materials synthesis, energy generation and storage). This symposium will highlight advances in analytical measurement strategies that are harnessing phenomena at electrified interfaces to achieve selectivity in sensing and separations and advance understanding of nanometer-scale phenomena that limit efficiency in renewable energy systems. Presentations will look in-depth at the latest approaches to electric field based separations and cell isolation to support disease diagnosis and treatment, platforms for selective sensing in femtoliter solution volumes and detecting turnover at individual catalytic sites, and techniques for imaging processes within buried electrified interfaces that are key to efficiency in chemical-to-electrical energy conversion. The speakers proposed are recipients of the Society for Electroanalytical Chemistry (SEAC) 2021 and 2022 awards and represent a balance of distinguished senior and early career investigators. The presentations will stimulate new ideas to help shape our understanding of electrified interfaces and their broad impacts in chemical analysis.