Recombinant Antibody Production
Manufacturing
Information
Transient gene expression (TGE) is the method of choice when it comes to fast production of recombinant antibodies in mammalian systems (HEK and CHO). Using transient expression, you can skip the time-consuming and labor-intensive creation of stable cell lines and receive milligram to gram amounts of your protein of interest within weeks.
Within years of research and development we established InVEST, our proprietary system for large-scale transient gene expression in HEK cells. InVEST is a highly optimized system which allows an increase in production up to 80-fold compared to standard systems.
InVivo is a well experienced service provider with more than 700 successfully completed TGE projects. We offer you custom recombinant antibody production, exactly tailored to your needs.
Service package includes:
cDNA synthesis (incl. codon optimization) and cloning into InVivo`s proprietary expression vector
Endotoxin-free plasmid preparation
Transient transfection of HEK cells
One-step purification via protein A affinity chromatography
QC services: UV280 nm, Capillary Gel Electrophoreses (CGE), analytical SEC
Guaranteed purity ≥ 90%
Deliverables:
40 mg of high-quality recombinant antibodies (IgG)
LC and HC in synthesis vectors (1-2 μg)
Certificate of Analysis
