Recombinant Protein Expression

Recombinant Protein Expression

CDMO

Information

We offer you custom recombinant protein production exactly tailored to your needs. With more than 700 successfully completed projects, InVivo is a well experienced service partner to get you started on your transient expression project. Using our proprietary system InVEST for large-scale transient gene expression in HEK cells, we deliver milligram to gram amounts of high-quality recombinant proteins in only a few weeks. Feasibility Study and Extended Feasibility Study: We conduct a small-scale pilot production in HEK cells to assess the feasibility to express and purify your protein of interest. Service package includes: -cDNA synthesis (incl. codon optimization), cloning into InVivo`s proprietary expression vector and endotoxin-free plasmid preparation -Small-scale transient transfection of HEK cells -Cultivation of 150 ml culture (Feasibility Study) or 1L culture (Extended Feasibility Study) -One-step purification -UV280 nm, CGE or SDS-PAGE Additional services: -Up to 100 small-scale (5-20 mg) expression assays in parallel (Target Discovery, Drug Screening, etc.) -Species and subclass switches -Protein mutations through amino acid exchanges -Tag cleavage Recombination protein Production (large scale) -Subsequent to the feasibility study, we offer you our large-scale production service for recombinant proteins. Using this service, you can receive milligram to gram amounts of your recombinant protein with a fixed unit price per mg protein.
InVivo BioTech Services GmbHInVivo is an ISO-certified German contract manufacturing organization (CMO) with more than 20 years of experience in cell culture and the development and production of monoclonal antibodies and recombinant proteins  for research, diagnostic and pre-clinical use. As an independent service provider, InVivo offers cost-effective outsourcing solutions plus a wide range of different expression systems to meet customer needs. We work with companies worldwide, including major diagnostic companies, research institutes and biopharmaceutical companies. We provide a complete range of antibody services, from antibody optimization and development to protein labelling. For monoclonal antibody production, our capacity ranges from  the milligram to the kilogram scale, and we have experience in the expression of antibodies from all kinds of subclasses and species. Besides mAB production, InVivo also focuses on the expression of recombinant proteins, including difficult-to-express proteins. Therefore we established a proprietary, highly-optimized expression system for transient gene expression in HEK cells, called InVest. This system combines an optimized host cell line, sophisticated vector design, large-scale plasmid preparation, serum-free cultivation media and a novel transfection reagent. InVivo now offers diverse SARS-CoV-2 Antigens in large-scale quantities, which are suitable for the development of highly sensitive IVD kits. Please visit our website for more information: https://www.invivo.de/cell-line-development/sars-cov-2-antigens/ For more details on our complete service portfolio please visit www.invivo.de

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