Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh resolution MALDI FT-ICR mass spectrometry

• The six subunits of a glycated BsAb were measured in a single ultrahigh-resolution MALDI FT-ICR mass spectrum, without the need of any separation
• Ultrahigh-resolution MALDI-in-source decay mass spectra were obtained from the simultaneous measurements of all (glycated) subunits
• MALDI-ISD analysis allowed us to localize hot-spot glycation sites that were not identified using a standard bottom-up strategy

Simone Nicolardi, Senior Researcher, Leiden University Medical Center